Mechanical Strains Induced in Osteoblasts by Use of Point Femtosecond Laser Targeting

Cranfield, Charles; Bomzon, Ze'ev; Day, Daniel; Gu, Min; Cartmell, Sarah

doi:https://doi.org/10.1155/IJBI/2006/21304

International Journal of Biomedical Imaging

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Volume 2006 | Article ID 021304 | https://doi.org/10.1155/IJBI/2006/21304

Mechanical Strains Induced in Osteoblasts by Use of Point Femtosecond Laser Targeting

Charles Cranfield,¹Ze'ev Bomzon,^1,2Daniel Day,¹Min Gu,¹and Sarah Cartmell³

Academic Editor: Lizhi Sun

Received11 May 2006

Revised03 Sept 2006

Accepted17 Sept 2006

Published06 Dec 2006

Abstract

A study demonstrating how ultrafast laser radiation stimulates osteoblasts is presented. The study employed a custom made optical system that allowed for simultaneous confocal cell imaging and targeted femtosecond pulse laser irradiation. When femtosecond laser light was focused onto a single cell, a rise in intracellular Ca2+ levels was observed followed by contraction of the targeted cell. This contraction caused deformation of neighbouring cells leading to a heterogeneous strain field throughout the monolayer. Quantification of the strain fields in the monolayer using digital image correlation revealed local strains much higher than threshold values typically reported to stimulate extracellular bone matrix production in vitro. This use of point targeting with femtosecond pulse lasers could provide a new method for stimulating cell activity in orthopaedic tissue engineering.

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Copyright

Copyright © 2006 Charles Cranfield et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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