Multispectral Bioluminescence Tomography: Methodology and Simulation

Cong, Alexander X.; Wang, Ge

doi:https://doi.org/10.1155/IJBI/2006/57614

International Journal of Biomedical Imaging

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Volume 2006 | Article ID 057614 | https://doi.org/10.1155/IJBI/2006/57614

Multispectral Bioluminescence Tomography: Methodology and Simulation

Alexander X. Cong¹and Ge Wang¹

Academic Editor: Jie Tian

Received18 Sept 2005

Accepted11 Nov 2005

Published05 Feb 2006

Abstract

Bioluminescent imaging has proven to be a valuable tool for monitoring physiological and pathological activities at cellular and molecular levels in living small animals. Using biological techniques, target cells can be tagged with reporters encoding several kinds of luciferase enzymes, which generate characteristic photons in a wide spectrum covering the infrared range. Part of the diffused light can reach the body surface of the small animal, be separated into several spectral bands using appropriate filters, and collected by a sensitive CCD camera. Here we present a bioluminescence tomography (BLT) method for a bioluminescent source reconstruction from multispectral data measured on the external surface, and demonstrate the advantages of multispectral BLT in a numerical study using a heterogeneous mouse chest phantom. The results show that the multispectral approach significantly improves the accuracy and stability of the BLT reconstruction even if the data are highly noisy.

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Copyright

Copyright © 2006 Alexander X. Cong and Ge Wang. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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