Synthesis and Bioconjugation of Gold Nanoparticles as Potential Molecular Probes for Light-Based Imaging Techniques

Rayavarapu, Raja Gopal; Petersen, Wilma; Ungureanu, Constantin; Post, Janine N.; van Leeuwen, Ton G.; Manohar, Srirang

doi:https://doi.org/10.1155/2007/29817

International Journal of Biomedical Imaging

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Recent Advances in Molecular Imaging

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Volume 2007 | Article ID 029817 | https://doi.org/10.1155/2007/29817

Synthesis and Bioconjugation of Gold Nanoparticles as Potential Molecular Probes for Light-Based Imaging Techniques

Raja Gopal Rayavarapu,¹Wilma Petersen,¹Constantin Ungureanu,¹Janine N. Post,²Ton G. van Leeuwen,¹and Srirang Manohar¹

Academic Editor: Wenxiang Cong

Received15 Jan 2007

Accepted14 Jun 2007

Published19 Aug 2007

Abstract

We have synthesized and characterized gold nanoparticles (spheres and rods) with optical extinction bands within the “optical imaging window.” The intense plasmon resonant driven absorption and scattering peaks of these nanoparticles make them suitable as contrast agents for optical imaging techniques. Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells. The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface. We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles. Some proposed applications of these potential molecular probes in the field of biomedical imaging are also discussed.

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Copyright

Copyright © 2007 Raja Gopal Rayavarapu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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