Development of a Confocal Optical System Design for Molecular Imaging Applications of Biochip

Huang, Guoliang; Xu, Shukuan; Zhu, Jiang; Deng, Cheng; Dong, Zhonghua; Yang, Yang; Yang, Xiaoyong; Wang, Xianhua; Jin, Guofan

doi:https://doi.org/10.1155/2007/79710

International Journal of Biomedical Imaging

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Recent Advances in Molecular Imaging

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Research Article | Open Access

Volume 2007 | Article ID 079710 | https://doi.org/10.1155/2007/79710

Development of a Confocal Optical System Design for Molecular Imaging Applications of Biochip

Guoliang Huang,^1,2Shukuan Xu,^1,2Jiang Zhu,^1,2Cheng Deng,^1,2Zhonghua Dong,^1,2Yang Yang,^1,2Xiaoyong Yang,²Xianhua Wang,²and Guofan Jin³

Academic Editor: Jie Tian

Received19 Apr 2007

Revised23 Jun 2007

Accepted15 Jul 2007

Published30 Aug 2007

Abstract

A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used. This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners. The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass. Some applications of gene and protein imagings using the dual laser confocal scanner are described.

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Copyright

Copyright © 2007 Guoliang Huang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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