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International Journal of Biomedical Imaging
Volume 2011, Article ID 236854, 13 pages
Research Article

Development of a New Tool for 3D Modeling for Regenerative Medicine

1Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Sezione di Biochimica e Biologia Molecolare, Università degli Studi di Perugia, Via del Giochetto, 06126 Perugia, Italy
2Translational Cardiomyology Laboratory, SCIL K.U. Leuven, 3000 Leuven, Belgium
3Dipartimento di Medicina Sperimentale, Sezione Anatomia Umana, Università di Pavia, 27100 Pavia, Italy
4Dipartimento di Medicina Sperimentale e Diagnostica, Sezione di Microbiologia, Università degli Studi di Ferrara, 44121 Ferrara, Italy
5Laboratorio di Neurogenetica, CERC-IRCCS Santa Lucia, Rome, Italy
6Dipartimento di Neuroscienze, Università di Roma “Tor Vergata”, Rome, Italy

Received 4 November 2010; Revised 4 March 2011; Accepted 6 April 2011

Academic Editor: Pingkun Yan

Copyright © 2011 Filippo Mattoli et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Video 1 and Video 2 showed the 3-D model of C57/BL6 mouse brain after the injection with HSVT0Z herpes simplex viral vector.

The model allowed the detection the τ-Galactosidase stained (the tonality of X-Gal staining is blue and always greater than the general tonality of the brain section which is grey) and the identification of the signal position in all the brain areas.

The 3-D model allows to appreciate the high distribution of the HSV-T0Z viral vector in brain area far-away from the site of injection, in both treated and untreated hemisphere and cerebellum (video.1).

Further, in this model it is possible to merge the signal with the brain anatomical structureallowing the investigation of putative mechanisms involving the transgenic distribution (video.1, video.2).

  1. Supplementary Material