Three-Dimensional Imaging of Pulmonary Fibrotic Foci at the Alveolar Scale Using Tissue-Clearing Treatment with Staining Techniques of Extracellular Matrix

<div>Three-dimensional images of collagen I stained using immunofluorescence in fibrotic lungs after Clear<sup>T2</sup> tissue-clearing treatment. (a) Three-dimensional images reconstructed from single-plane images. Each 5 <i>μ</i>m stack is 20–120 <i>μ</i>m below the lung surface. (b) The image of cross-sections indicated by the red lines in (a). (c) Single-plane images at the indicated depth (<i>μ</i>m) from the lung surface. Green fluorescence indicates the localization of collagen I. The scale bar is 100 <i>μ</i>m.</div>

International Journal of Biomedical Imaging

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Figure 5

Figure 5: Three-Dimensional Imaging of Pulmonary Fibrotic Foci at the Alveolar Scale Using Tissue-Clearing Treatment with Staining Techniques of Extracellular Matrix 

Figure 5 | Three-Dimensional Imaging of Pulmonary Fibrotic Foci at the Alveolar Scale Using Tissue-Clearing Treatment with Staining Techniques of Extracellular Matrix 