Elastic Membrane That Undergoes Mechanical Deformation Enhances Osteoblast Cellular Attachment and Proliferation
Figure 7
Phalloidin-stained actin filaments of osteoblasts grown on silicone membranes. MC3T3-E1 osteoblast cells were seeded on FN-coated silicone membrane and subjected to equibiaxial strain for 2 h. Cells were treated with rhodamine-labeled phalloidin (1 : 100) and then visualized by confocal microscopy. (a) Cells on: nonstretched UVO-activated FN-coated silicone membrane; (b) Cells on: stretched, 30 min UVO-activated FN-coated silicone membrane. Note the slightly elevated fluorescence of the actin filaments in the stretched sample.
