The Effect of Laminin-1-Doped Nanoroughened Implant Surfaces: Gene Expression and Morphological Evaluation

Figure 3

Gene expressions of bone formation markers by real-time RT-PCR for the non-laminin-1-coated (control) and -coated (test) groups. After 2 weeks, the surrounding tissues of implants were collected and total RNA of pooled samples was isolated. The osteogenic markers (ALP, osteocalcin, Runx2, calcitonin receptor, collagen I, TRAP, IGF-1, and ATPase) and inflammation markers (IL-6, IL-10, and TNF-α) were evaluated and higher values were detected for the experimental group. The relative expressions of target genes were normalized with housekeeping gene β-actin.