Optimization of Human Corneal Endothelial Cells for Culture: The Removal of Corneal Stromal Fibroblast Contamination Using Magnetic Cell Separation
Figure 1
(a) Representative micrograph of a confluent homogeneous monolayer of successfully isolated human corneal endothelial cells at Day 14. (b) Representative micrograph of a failed isolation attempt resulting in stromal contamination at Day 7. The cellular boundary between the polygonal corneal endothelial cells (*) and the confluent elongated corneal stromal fibroblasts (†) can be clearly defined (scale bars = 100 μm).
