Research Article

Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs

Figure 3

BMP2 production in microencapsulated MSCs. (a) Cells were transduced with 15,000 vp/cell adenoviral BMP2 prior to microencapsulation and plated out freshly or cryopreserved. (+, # ), (b) ovine A and ovine B cryopreservation and BMP2 transduction effect on MSC viability on day of microencapsulation (day of thaw for cryopreserved samples), and (c) 4 days after microencapsulation ( ).
861794.fig.003a
(a) BMP2 production from microbeads
861794.fig.003b
(b) MSC viability on day of encapsulation
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(c) MSC viability 4 days following encapsulation