Cellular Nutrition in Complex Three-Dimensional Scaffolds: A Comparison between Experiments and Computer Simulations

Figure 6

Static cell culture in the in vitro 3D module. Fluorescence (left) and FESEM images (right) of MG-63 cells after culturing for 7 days. Lower cell density and dead cells could be detected in the core region (levels 2 and 3). Fluorescence images: nuclei of vital cells = green, nuclei of dead cells = red (Axio Scope.A1, magnification 10x, bar 100 μm) and FESEM images: Ta = white, cells = black (FESEM SUPRA 25, magnification 50x, bar 100 μm).