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International Journal of Biomaterials
Volume 2016 (2016), Article ID 9825659, 14 pages
http://dx.doi.org/10.1155/2016/9825659
Research Article

Preparation and Evaluation of Gelatin-Chitosan-Nanobioglass 3D Porous Scaffold for Bone Tissue Engineering

1Department of Ceramic Engineering, National Institute of Technology, Rourkela 769008, India
2Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela 769008, India

Received 31 July 2015; Revised 18 November 2015; Accepted 23 November 2015

Academic Editor: Esmaiel Jabbari

Copyright © 2016 Kanchan Maji et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. M. Stevens and J. H. George, “Exploring and engineering the cell surface interface,” Science, vol. 310, no. 5751, pp. 1135–1138, 2005. View at Publisher · View at Google Scholar · View at Scopus
  2. S. Gronthos, P. J. Simmons, S. E. Graves, and P. G. Robey, “Integrin-mediated interactions between human bone marrow stromal precursor cells and the extracellular matrix,” Bone, vol. 28, no. 2, pp. 178–181, 2001. View at Publisher · View at Google Scholar · View at Scopus
  3. J. D. Chen, Y. J. Wang, K. Wei, S. H. Zhang, and X. T. Shi, “Self-organization of hydroxyapatite nanorods through oriented attachment,” Biomaterials, vol. 28, no. 14, pp. 2275–2280, 2007. View at Publisher · View at Google Scholar · View at Scopus
  4. Y. Wang, C. Yang, X. Chen, and N. Zhao, “Development and characterization of novel biomimetic composite scaffolds based on bioglass-collagen-hyaluronic acid-phosphatidylserine for tissue engineering applications,” Macromolecular Materials and Engineering, vol. 291, no. 3, pp. 254–262, 2006. View at Publisher · View at Google Scholar · View at Scopus
  5. C. V. M. Rodrigues, P. Serricella, A. B. R. Linhares et al., “Characterization of a bovine collagen-hydroxyapatite composite scaffold for bone tissue engineering,” Biomaterials, vol. 24, no. 27, pp. 4987–4997, 2003. View at Publisher · View at Google Scholar · View at Scopus
  6. S. Mann, “Molecular tectonics in biomineralization and biomimetic materials chemistry,” Nature, vol. 365, no. 6446, pp. 499–505, 1993. View at Publisher · View at Google Scholar · View at Scopus
  7. J. Rich, T. Jaakkola, T. Tirri, T. Närhi, A. Yli-Urpo, and J. Seppälä, “In vitro evaluation of poly(epsilon-caprolactone-co-DL-lactide)/bioactive glass composites,” Biomaterials, vol. 23, no. 10, pp. 2143–2150, 2002. View at Publisher · View at Google Scholar · View at Scopus
  8. D. Walsh, T. Furuzono, and J. Tanaka, “Preparation of porous composite implant materials by in situ polymerization of porous apatite containing ε-caprolactone or methyl methacrylate,” Biomaterials, vol. 22, no. 11, pp. 1205–1212, 2001. View at Publisher · View at Google Scholar · View at Scopus
  9. Q.-Q. Qiu, P. Ducheyne, and P. S. Ayyaswamy, “New bioactive, degradable composite microspheres as tissue engineering substrates,” Journal of Biomedical Materials Research, vol. 52, no. 1, pp. 66–76, 2000. View at Publisher · View at Google Scholar · View at Scopus
  10. Y. Shikinami and M. Okuno, “Bioresorbable devices made of forged composites of hydroxyapatite (HA) particles and poly-L-lactide (PLLA). Part II: practical properties of miniscrews and miniplates,” Biomaterials, vol. 22, pp. 3197–3211, 2001. View at Google Scholar
  11. J. E. Devin, M. A. Attawia, and C. T. Laurencin, “Three-dimensional degradable porous polymer-ceramic matrices for use in bone repair,” Journal of Biomaterials Science, Polymer Edition, vol. 7, no. 8, pp. 661–669, 1996. View at Publisher · View at Google Scholar · View at Scopus
  12. X. Deng, J. Hao, and C. Wang, “Preparation and mechanical properties of nanocomposites of poly(D,L-lactide) with Ca-deficient hydroxyapatite nanocrystals,” Biomaterials, vol. 22, no. 21, pp. 2867–2873, 2001. View at Publisher · View at Google Scholar · View at Scopus
  13. S. C. Rizzi, D. J. Heath, A. G. A. Coombes, N. Bock, M. Textor, and S. Downes, “Biodegradable polymer/hydroxyapatite composites: surface analysis and initial attachment of human osteoblasts,” Journal of Biomedical Materials Research, vol. 55, no. 4, pp. 475–486, 2001. View at Google Scholar · View at Scopus
  14. S. K. L. Levengood and M. Q. Zhang, “Chitosan-based scaffolds for bone tissue engineering,” Journal of Materials Chemistry B, vol. 2, no. 21, pp. 3161–3184, 2014. View at Publisher · View at Google Scholar · View at Scopus
  15. F. Croisier and C. Jérôme, “Chitosan-based biomaterials for tissue engineering,” European Polymer Journal, vol. 49, no. 4, pp. 780–792, 2013. View at Publisher · View at Google Scholar · View at Scopus
  16. S. K. Nandi, B. Kundu, and D. Basu, “Protein growth factors loaded highly porous chitosan scaffold: a comparison of bone healing properties,” Materials Science and Engineering C, vol. 33, no. 3, pp. 1267–1275, 2013. View at Publisher · View at Google Scholar · View at Scopus
  17. P. Ghosh, A. P. Rameshbabu, N. Dogra, and S. Dhara, “2,5-dimethoxy 2,5-dihydrofuran crosslinked chitosan fibers enhance bone regeneration in rabbit femur defects,” RSC Advances, vol. 4, no. 37, pp. 19516–19524, 2014. View at Publisher · View at Google Scholar · View at Scopus
  18. Y. Shen, Y. Zhan, J. Tang et al., “Multifunctioning pH-responsive nanoparticle from hierarchical self-assembly of polymer brush for cancer drug delivery,” AIChE Journal, vol. 54, pp. 2979–2989, 2008. View at Google Scholar
  19. J. P. Vacanti and R. Langer, “Tissue engineering: the design and fabrication of living replacement devices for surgical reconstruction and transplantation,” The Lancet, vol. 354, no. 1, pp. 32–34, 1999. View at Google Scholar · View at Scopus
  20. H. Nagahama, H. Maeda, T. Kashiki, R. Jayakumar, T. Furuike, and H. Tamura, “Preparation and characterization of novel chitosan/gelatin membranes using chitosan hydrogel,” Carbohydrate Polymers, vol. 76, no. 2, pp. 255–260, 2009. View at Publisher · View at Google Scholar · View at Scopus
  21. P. J. Vandevord, H. W. T. Matthew, S. P. Desilva, L. Mayton, B. Wu, and P. H. Wooley, “Evaluation of the biocompatibility of a chitosan scaffold in mice,” Journal of Biomedical Materials Research, vol. 59, no. 3, pp. 585–590, 2002. View at Publisher · View at Google Scholar · View at Scopus
  22. M. Gravel, T. Gross, R. Vago, and M. Tabrizian, “Responses of mesenchymal stem cell to chitosan-coralline composites microstructured using coralline as gas forming agent,” Biomaterials, vol. 27, no. 9, pp. 1899–1906, 2006. View at Publisher · View at Google Scholar · View at Scopus
  23. J. S. Mao, L. G. Zhao, Y. J. Yin, and K. D. Yao, “Structure and properties of bilayer chitosan-gelatin scaffolds,” Biomaterials, vol. 24, no. 6, pp. 1067–1074, 2003. View at Publisher · View at Google Scholar · View at Scopus
  24. T. Chen, H. D. Embree, E. M. Brown, M. M. Taylor, and G. F. Payne, “Enzyme-catalyzed gel formation of gelatin and chitosan: potential for in situ applications,” Biomaterials, vol. 24, no. 17, pp. 2831–2841, 2003. View at Publisher · View at Google Scholar · View at Scopus
  25. J. Mao, L. Zhao, K. de Yao, Q. Shang, G. Yang, and Y. Cao, “Study of novel chitosan-gelatin artificial skin in vitro,” Journal of Biomedical Materials Research A, vol. 64, no. 2, pp. 301–308, 2003. View at Google Scholar · View at Scopus
  26. W. Xia, W. Liu, L. Cui et al., “Tissue engineering of cartilage with the use of chitosan-gelatin complex scaffolds,” Journal of Biomedical Materials Research B, vol. 71, no. 2, pp. 373–380, 2004. View at Publisher · View at Google Scholar · View at Scopus
  27. Y. Yin, F. Ye, J. Cui, F. Zhang, X. Li, and K. Yao, “Preparation and characterization of macroporous chitosan-gelatin/beta-tricalcium phosphate composite scaffolds for bone tissue engineering,” Journal of Biomedical Materials Research Part A, vol. 67, no. 3, pp. 844–855, 2003. View at Google Scholar
  28. L. L. Hench, R. J. Splinter, W. C. Allen, and T. K. Greenlee, “Bonding mechanisms at the interface of ceramic prosthetic materials,” Journal of Biomedical Materials Research, vol. 5, no. 6, pp. 117–141, 1971. View at Publisher · View at Google Scholar
  29. H. Oudadesse, M. Mami, R. Doebez-Sridi et al., “Study of various mineral compositions and their bioactivity of bioactive glasses,” Bioceramics, vol. 22, pp. 379–382, 2009. View at Google Scholar
  30. L. L. Hench, “The story of bioglass,” Journal of Materials Science: Materials in Medicine, vol. 17, no. 11, pp. 967–978, 2006. View at Publisher · View at Google Scholar · View at Scopus
  31. L. L. Hench and J. K. West, “Biological applications of bioactive glasses,” Life Chemistry Reports, vol. 13, pp. 187–241, 1996. View at Google Scholar
  32. K. T. Shalumon, S. Sowmya, D. Sathish, K. P. Chennazhi, S. V. Nair, and R. Jayakumar, “Effect of incorporation of nanoscale bioactive glass and hydroxyapatite in PCL/chitosan nanofibers for bone and periodontal tissue engineering,” Journal of Biomedical Nanotechnology, vol. 9, no. 3, pp. 430–440, 2013. View at Publisher · View at Google Scholar · View at Scopus
  33. P. Gentile, M. Mattioli-Belmonte, V. Chiono et al., “Bioactive glass/polymer composite scaffolds mimicking bone tissue,” Journal of Biomedical Materials Research Part A, vol. 100, no. 10, pp. 2654–2667, 2012. View at Publisher · View at Google Scholar · View at Scopus
  34. M. Peter, N. S. Binulal, S. Soumya et al., “Nanocomposite scaffolds of bioactive glass ceramic nanoparticles disseminated chitosan matrix for tissue engineering applications,” Carbohydrate Polymers, vol. 79, no. 2, pp. 284–289, 2010. View at Publisher · View at Google Scholar · View at Scopus
  35. M. Peter, N. S. Binulal, S. V. Nair, N. Selvamurugan, H. Tamura, and R. Jayakumar, “Novel biodegradable chitosan-gelatin/nano-bioactive glass ceramic composite scaffolds for alveolar bone tissue engineering,” Carbohydrate Polymers, vol. 80, pp. 687–694, 2010. View at Google Scholar
  36. I. D. Xynos, M. V. J. Hukkanen, J. J. Batten, L. D. Buttery, L. L. Hench, and J. M. Polak, “Bioglass 45S5 stimulates osteoblast turnover and enhances bone formation in vitro: implications and applications for bone tissue engineering,” Calcified Tissue International, vol. 67, no. 4, pp. 321–329, 2000. View at Publisher · View at Google Scholar · View at Scopus
  37. R. C. Bielby, R. S. Pryce, L. L. Hench, and J. M. Polak, “Enhanced derivation of osteogenic cells from murine embryonic stem cells after treatment with ionic dissolution products of 58S bioactive sol-gel glass,” Tissue Engineering, vol. 11, no. 3-4, pp. 479–488, 2005. View at Publisher · View at Google Scholar · View at Scopus
  38. H.-Y. Li, R.-L. Du, and J. Chang, “Fabrication, characterization, and in vitro degradation of composite scaffolds based on PHBV and bioactive glass,” Journal of Biomaterials Applications, vol. 20, no. 2, pp. 137–155, 2005. View at Publisher · View at Google Scholar · View at Scopus
  39. C. García, S. Ceré, and A. Durán, “Bioactive coatings prepared by sol–gel on stainless steel 316L,” Journal of Non-Crystalline Solids, vol. 348, pp. 218–224, 2004. View at Publisher · View at Google Scholar · View at Scopus
  40. L. L. Hench and J. Wilson, An Introduction to Bioceramic, World Scientific, Singapore, 1993.
  41. K. Maji, S. Dasgupta, B. Kundu, and A. Bissoyi, “Development of gelatin-chitosan-hydroxyapatite based bioactive bone scaffold with controlled pore size and mechanical strength,” Journal of Biomaterials Science, Polymer Edition, vol. 26, no. 16, pp. 1190–1209, 2015. View at Publisher · View at Google Scholar
  42. K. Maji and S. Dasgupta, “Hydroxyapatite-chitosan and gelatin based scaffold for bone tissue engineering,” Transactions of the Indian Ceramic Society, vol. 73, no. 2, pp. 110–114, 2014. View at Publisher · View at Google Scholar · View at Scopus
  43. F.-L. Mi, “Synthesis and characterization of a novel chitosan-gelatin bioconjugate with fluorescence emission,” Biomacromolecules, vol. 6, no. 2, pp. 975–987, 2005. View at Publisher · View at Google Scholar · View at Scopus
  44. Q.-X. Niu, C.-Y. Zhao, and Z.-A. Jing, “An evaluation of the colorimetric assays based on enzymatic reactions used in the measurement of human natural cytotoxicity,” Journal of Immunological Methods, vol. 251, no. 1-2, pp. 11–19, 2001. View at Publisher · View at Google Scholar · View at Scopus
  45. S. R. Federman, V. C. Costa, D. C. L. Vasconcelos, and W. L. Vasconcelos, “Sol-gel SiO2-CaO-P2O5 biofilm with surface engineered for medical application,” Materials Research, vol. 10, no. 2, pp. 177–181, 2007. View at Google Scholar · View at Scopus
  46. Y. J. Yin, F. Zhao, X. F. Song, K. D. Yao, W. W. Lu, and J. G. Leong, “Preparation and characterization of hydroxyapatite/chitosan-gelatin network composite,” Journal of Applied Polymer Science, vol. 77, no. 13, pp. 2929–2938, 2000. View at Publisher · View at Google Scholar
  47. S. Itoh, M. Kikuchi, Y. Koyama et al., “Development of a novel biomaterial, hydroxyapatite/collagen (HAp/Col) composite for medical use,” Bio-Medical Materials and Engineering, vol. 15, no. 1-2, pp. 29–41, 2005. View at Google Scholar · View at Scopus
  48. M. Kikuchi, H. N. Matsumoto, T. Yamada, Y. Koyama, K. Takakuda, and J. Tanaka, “Glutaraldehyde cross-linked hydroxyapatite/collagen self-organized nanocomposites,” Biomaterials, vol. 25, no. 1, pp. 63–69, 2004. View at Publisher · View at Google Scholar · View at Scopus
  49. Y. Kuboki, H. Takita, D. Kobayashi et al., “BMP-induced osteogenesis on the surface of hydroxyapatite with geometrically feasible and nonfeasible structures: topology of osteogenesis,” Journal of Biomedical Materials Research, vol. 39, no. 2, pp. 190–199, 1998. View at Publisher · View at Google Scholar · View at Scopus
  50. N. Arabi and A. Zamanian, “Effect of cooling rate and gelatin concentration on the microstructural and mechanical properties of ice template gelatin scaffolds,” Biotechnology and Applied Biochemistry, vol. 60, no. 6, pp. 573–579, 2013. View at Publisher · View at Google Scholar · View at Scopus
  51. J. M. Oliveira, M. T. Rodrigues, S. S. Silva et al., “Novel hydroxyapatite/chitosan bilayered scaffold for osteochondral tissue-engineering applications: scaffold design and its performance when seeded with goat bone marrow stromal cells,” Biomaterials, vol. 27, no. 36, pp. 6123–6137, 2006. View at Publisher · View at Google Scholar · View at Scopus