Metabolic Engineered Biocatalyst: A Solution for PLA Based Problems

Table 2

Table summarizes genetically manipulated microorganisms used in PLA production.

Sr. No.	Name of Microorganism	Genetic Manipulation	Substrate Utilized	Yield of PLA	Analytical Technique
1	Escherichia coli[14]	Insertion of propionate CoA-transferase and polyhydroxyalkanoate (PHA) synthase gene[15]	Glucose [14]	43 wt% [14]	Gas Chromatography[14]
2	Escherichia coli[16]	Insertion of propionate CoA-transferase and polyhydroxyalkanoate (PHA) synthase gene [16]; Knocking out the ackA, ppc, and adhE genes[16]; Replacing the promoters of the ldhA and acs genes with the trc promoter[16]	56 wt% from glucose[16]	55-86 mol%[16]	Gas Chromatography[16]
3.	Escherichia coli[17]	Introduction of propionate; CoA transferase (PctCp) gene from Clostridium propionicum; And 19 polyhydroxyalkanoate (PHA) synthase 1 (PhaC1Ps6-19) from Pseudomonas sp.; into Escherichia coli for the generation of; lactyl-CoA endogenously and incorporation of lactyl-CoA[17]	62wt% glucose[17]	20–49 mol%[17]	Gas Chromatography[17]
4.	Escherichia coli[18]	introduction of heterologous pathways having engineered propionate CoA-transferase and polyhydroxyalkanoate (PHA) synthase in to E. coli for generation of lactyl-CoA [18]	46 wt% glucose[18]	70mol% [18]	Gas Chromatography[17]