Verification of the Functional Antioxidant Activity and Antimelanogenic Properties of Extracts of Poria cocos Mycelium Fermented with Freeze-Dried Plum Powder
Figure 3
(a) Cytotoxicity of extract on mouse B16 melanoma cells. B16F0 cell line was treated with extracts for 24 hours and cell cytotoxicity was determined by CCK-8 assay. Culture supernatants were removed, and cell counting Kit-8 (CCK-8) was added. (b) Inhibitory effects of extract on the activity of tyrosinase. The lysates of B16F0 melanoma cells containing tyrosinase were incubated with DOPA for 1 h. Tyrosinase activity was measured as described in the Material and Methods. (c) Inhibitory effects of extract on the melanin synthesis in B16F0 melanoma cells. The cells were cultured in the presence of the extracts at concentration of 1000 μg/mL for 48 h. The determination of melanin content was measured as described in the Materials and Methods. The results were expressed as the average of triplicate experiments. Data is expressed as a ratio of the control as mean±S.D of 3 separate experiments. +p<0.05, ++p<0.01 or +++p<0.001 Medium group vs. a-MSH group. p<0.05, p<0.01 or p<0.001 a-MSH group vs. Sample group. #p<0.05, ##p<0.01 or ###p<0.001 1%PP group vs. PC, and PPE group.