Table 1: Hsp90 regulation level after silencing in U87-MG cells.

SamplesKinase activity for 1 μg Protein Hsp90α expression [%]

U87-MG (Control)234.51 ± 476 ± 0.7
U87-MG-17AAG44.53 ± 0.1**42.7 ± 0.2**
U87-MG-shRNA hsp90α95.16 ± 1.9**64.1 ± 1.4**

Data values are mean ± standard deviation, ; and are considered to be statistically significant, with * being significant and ** being very significant.
Note: Hsp90 protein levels were determined by FACS analysis, and Akt/PKB kinase activities were measured in the control (wild-type U87-MG cells), treated cells (U87-MG-shhsp90α and U87-MG-17AAG), and its activity.