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International Journal of Cell Biology
Volume 2009, Article ID 273651, 8 pages
Research Article

The Response of Creatine Kinase Specific Activity in Rat Pituitary to Estrogenic Compounds and Vitamin D Less-Calcemic Analogs

1Institute of Endocrinology, Metabolism and Hypertension, Sourasky Medical Center, Tel-Aviv 64239, Israel
2The Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 64239, Israel
3Faculty of Science, University of Haifa, Har-Hacarmel, Haifa 31905, Israel
4Laboratory of Natural Compounds for Medical Use, Migal-Galilee Technological Center, Kiryat- Shmona 10200, Israel
5Department of Chemistry, The Johns Hopkins University, Baltimore, MD 21218, USA

Received 20 April 2009; Accepted 24 June 2009

Academic Editor: Gary S. Stein

Copyright © 2009 D. Somjen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We examined the response of rat female pituitary at different metabolic stages to treatments with estrogenic compounds and vitamin D analogs. Immature or ovariectomized (Ovx) female rats responded by increased creatine kinase specific activity (CK) to estradiol-17 ( ), genistein (G), daidzein (D), biochainin A (BA), quecertin (Qu), carboxy- G (cG), carboxy- BA (cBA), and raloxifene (Ral). The response was inhibited when Ral was injected together with the estrogens. CK was increased when hormones were injected daily into Ovx rats for 4 different time periods. Pretreatment with the less-calcemic vitamin D analogs JK 1624 -2 (JKF) or QW 1624 -2 (QW) followed by estrogenic injection resulted in increased response and sensitivity to and loss of inhibition of by Ral. CK was also increased by feeding with or licorice or its components dose- and time- dependent in immature or Ovxrats. Diabetic female rats did not respond to increased doses of . In conclusion, rat female pituitary is estrogens-responsive organ, suggesting to considerits response for HRT in postmenopausal women for both beneficial and hazardous aspects.