Alteration of Sarcoplasmic Reticulum Ca²⁺ Release in Skeletal Muscle from Calpain 3-Deficient Mice

Table 1

Quantification of the expression of calpain 3 transcripts in wild type myoblasts and myotubes. These data were obtained from the gel electrophoresis of the RT-PCR reactions using the primer pairs p94sys3, p94sys5 and p94sys6 on murine myoblast (MB) or myotube (MT) mRNA (see Figure 1(b)). The gel and the corresponding quantification are representative of 3 different experiments where similar results were observed. These results have to be taken qualitatively since the experiments were performed by classical RT-PCR and not by quantitative RT-PCR. Numbers are given in arbitrary units.

Primer	p94sys3		p94sys5		p94sys6
Cell type	MB	MT	MB	MT	MB	MT
Band 1	43596	165378	155681	205283	66937	46845
Band 2	207433	89706	73219	69370	45719
Band 3					43022
Total	251029	255084	228900	274653	155678	46845