Cell Cycle-Dependent Localization of Voltage-Dependent Calcium Channels and the Mitotic Apparatus in a Neuroendocrine Cell Line(AtT-20)
Figure 3
Calcium chelation with fura PE3/AM interrupts specific stages of mitosis. Addition of Fura PE3/AM (M) results in a cessation and/or regression of mitotic stages prophase (b) and metaphase (d). However, progression from anaphase to cytokinesis is unaffected (f). (a), (c) and (e) are controls for comparison; images are captured at 30-second intervals as described in Methods; times for these images are as indicated in lower right of each panel. In the control cell (a) prometaphase proceeds to metaphase within 51 minutes and continues to cytokinesis within 1 minute 18 seconds. In contrast, in the Fura PE3/AM-treated prometaphase cell (b), chromosomes decondense and no mitotic progression is seen in minutes. Similarly, in the presence of Fura PE3/AM, metaphase cells show chromosomes aligned but do not progress in minutes (d). In the presence of Fura PE3/AM, anaphase progresses normally to cytokinesis (f).
