Cell Cycle-Dependent Localization of Voltage-Dependent Calcium Channels and the Mitotic Apparatus in a Neuroendocrine Cell Line(AtT-20)

<table><i>Sites of calcium transients during mitosis</i>. Calcium transients correspond  with  sites of CaV1 immunofluorescence in AtT-20 Cells during mitosis. Simultaneous imaging of (a) Fluo4 (<svg height="13.55" id="M32" style="vertical-align:-2.29482pt" version="1.1" viewbox="0 0 20.0625 13.55" width="20.0625" xmlns="http://www.w3.org/2000/svg">
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<tspan style="font-size: 12.50px; " x="0" y="0">4</tspan>
<tspan style="font-size: 12.50px; " x="8.3269978" y="0">𝜇</tspan>
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</svg>M) intracellular calcium transients and (b) cell morphology by DIC in AtT-20 cells during cytokinesis.  Regions: 1=midbody; 2, 3=spindle mid-zone; 4=nuclear region of interphase cell; 5=background (media only). (c) Plot of fluorescence units for each region over time (sec). Regions of enhanced calcium transients correspond to midbody (1) and spindle mid-zone (2, 3).  Break in X-axis marks time of acquisition following loading of Fluo-4.</table>

International Journal of Cell Biology

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Figure 4

Figure 4: Cell Cycle-Dependent Localization of Voltage-Dependent Calcium Channels and the Mitotic Apparatus in a Neuroendocrine Cell Line(AtT-20) 