Ret Finger Protein: An E3 Ubiquitin Ligase Juxtaposed to the XY Body in Meiosis

Figure 2

RFP defines a region underlying the XY body in pachytene spermatocyte. Immunofluorescent labeling of RFP on seminiferous tubule ((a), bar, 20 𝜇m) or in pachytene spermatocytes (b), (c). Coimmunolocalization of RFP and 𝛾H2AX (d), (e) and RFP and SUMO-I (f), (g) in pachytene spermatocytes (bar, 8 𝜇m). In pachytene spermatocytes, RFP immunofluorescent staining (green) is condensed in a cap-like structure localized at the border of the nucleus (DAPI, blue). The XY body is visualized either by 𝛾H2AX or SUMO-I antibodies (red). Superposition of labeling is never observed. The lace structure of RFP is evidenced when seen full face (g).