Optimum Combination of Insulin-Transferrin-Selenium and Fetal Bovine Serum for Culture of Rabbit Articular Chondrocytes in Three-Dimensional Alginate Scaffolds

Figure 4

Agarose gel electrophoresis of the PCR products obtained from chondrocyte-seeded alginate hydrogel cultured in (a, e) medium supplemented with 1% ITS plus 2% FBS, (b, f) medium supplemented with 1% ITS plus 4% FBS, (c, g) medium supplemented with 1% ITS plus 8% FBS, or (d, h) medium with 10% FBS. Three target genes, aggrecan, type I, and II collagens were examined, while GAPDH was used as a housekeeping gene. Samples for lane a–d were cultured for 1 week and for lane e–h were cultured for 3 weeks.