Determinants Present in the Receptor Carboxy Tail Are Responsible for Differences in Subtype-Specific Coupling of -Adrenergic Receptors to Phosphoinositide 3-Kinase
Figure 6
Akt activity with chimeric β-AR stimulation. HEK 293 cells were
transfected with
-AR or
-AR. Forty-eight hours after the
transfection, cells were stimulated with 1 μM isoproterenol for the indicated
times. Akt activity was determined by measuring levels of phosphorylated Akt
compared with total Akt produced by the stimulation of
-AR () or
-AR (■) expressing cells. Upper panel is a
representative immunoblot of the experiments (). Data from
these experiments is quantitated in the bottom panel as described in Section
2.
versus 0 minute.
