Evaluation of X-Inactivation Status and Cytogenetic Stability of Human Dermal Fibroblasts after Long-Term Culture

<table><i>Immunocytochemical (ICC) analysis of the number of inactive X chromosomes in HDF cultures.</i>  HDFs in passages 1, 5, and 10 showed one positively stained H3K27me3 punctate signal (arrow) in most nuclei. Two green signals (arrows), inactive of two inactive Xs, were observed in few of HDF cells at passage 10.</table>

International Journal of Cell Biology

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Figure 2: Evaluation of X-Inactivation Status and Cytogenetic Stability of Human Dermal Fibroblasts after Long-Term Culture 