Evaluation of X-Inactivation Status and Cytogenetic Stability of Human Dermal Fibroblasts after Long-Term Culture

Figure 3

XIST RNA FISH analysis of the number of inactive X chromosomes. FISH analysis of HDF cultures in passages 1, 5, and 10 showed that most of the nuclei counterstained with DAPI (in blue) have one small spot signal for XIST RNA (in red) in the merged image. This was in accordance with the results of the H3K27me3 ICC analysis in Figure 2.