The Small Heat Shock Protein HSP25/27 (HspB1) Is Abundant in Cultured Astrocytes and Associated with Astrocytic Pathology in Progressive Supranuclear Palsy and Corticobasal Degeneration
Figure 4
Colocalization of HSP27 with tau-immunoreactive inclusions. (b) Adjacent sections from affected brains, as indicated on the left, were double labelled for HSP27 (green) and PHF-1 tau (red). HSP27 colocalizes with a subset of astrocytic pathology. In PSP (a)–(f) astrocytic tufts positive for HSP27 and PHF-1 are seen (arrows) and in CBD (g)–(i), PHF-1 positive astrocytic plaques with HSP27 in the center are prominent (arrows). Scale bar, 25 m. Nuclei are stained with DAPI in the overlay images. (c) Confocal overlay images of representative cells from brain sections as depicted in (a) are shown (a,b, PSP patient 3; c, CBD patient 6). (d) Brain sections were double labelled for HSP27 (green) and GFAP (red). All cells positively stained for HSP27 were GFAP positive, demonstrating their astrocytic origin. Overlay images are shown for PSP patient 1 (a) and CBD patient 6 (b) and (c). Nuclei are stained with DAPI. Scale bar, 20 m.
