Research Article

Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro

Figure 4

Transcytosis of leptin in knockdown cells. Cells were infected with lentiviruses containing plKO.1, C7, or C9 shRNA. Leptin was added to the apical medium of C7 and C9 infected cells and leptin intracellular content (I-Lep) as well as leptin concentrations in the basal medium (B-Lep) were assessed. Results of the invalidations on the long (219.1) and short (219.3) isoforms were analyzed by RT-PCR (a) and Western blot (b). (c) Infection with the C7-virus decreased expression of the long isoform ( 48%) (c1) without affecting significantly the short one, and decreased I-Lep ( 43%) and B-Lep ( 58%) (c2). (d) C9-virus decreased both long and short isoforms, respectively by 47% and 45% (d1), and decreased I-Lep ( 57%) and B-Lep ( 68%) (d2). Bars represent the mean value s.e.m of 6 independent experiments.
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