529851.fig.005
Figure 5: Release of KCl-stimulated 3H-GABA: effect of cannabinoid agonist in control and acute stressed rats. Micro dissected EPL was incubated with 0.1 μM 3H-GABA and superfused with Krebs bicarbonate; after of 49 min of superfusion, 15 mM of KCl was added for 4 min. The cannabinoid agonist Win 1 μM or methanandamide 10 μM (AEA) was added 14 min before, during, and 12 min after KCl stimulation. Rats subjected to 1 hr. immobilization stress (Acute stress: AS) were sacrificed and olfactory bulb immediately used for experiments. Results are expressed as a ratio of the % of 3H-GABA released as described in methods. * compared to control using Mann Whitney test.