PAI-1 Expression Is Required for HDACi-Induced Proliferative Arrest in ras-Transformed Renal Epithelial Cells

Figure 4

Summary characteristics of the NaB-induced phenotype in v-ras-transformed renal epithelial cells. NaB initiated a rapid cytostasis evident within 3 days after exposure with the acquisition of increased cell-spread area consistent with FALS assessment of cell size (i.e., Figure 2). Cells remained growth arrested even after protracted treatment (e.g., 46 days) although such long-term cultures had an increased binucleate frequency. PAI-1 mRNA/protein expression in control populations was low to undetectable in contrast to the levels of PAI-1 transcripts and protein evident in response to NaB. Plots illustrate the mean ± standard deviation for triplicate assessments for each of the parameters evaluated.