Cells expressing the Atg14-ΔC variant accumulate smaller autophagic bodies. (a) Electron micrographs of autophagic bodies accumulated in BJ2168 cells lacking vacuolar proteases. Cells were cultured for 3.5 h in nitrogen-depleted medium, and the accumulated autophagic bodies (arrow) were observed under an electron microscope. Bar represents 500 nm. (b) Average size of the autophagic body. Autophagic bodies with clear outlines, that is, sliced vertically at the position of the maximum diameter, were chosen, and their diameters were measured. The average autophagic body diameters were 558 nm (), 567 nm (), and 377 nm () in WT, Atg14-FL, and Atg14-ΔC cells, respectively. Bars indicate standard deviations. (c) Frequency of autophagic bodies with each diameter.
