Transplantable Subcutaneous Hepatoma 22a Affects Functional Activity of Resident Tissue Macrophages in Periphery
Figure 1
Dynamics of functional activity of peritoneal macrophages from mice bearing hepatoma 22a. -axis: time after inoculation of the tumor, days. -axis: (a) Spontaneous NO2-production by macrophages from ○—control mice and ⚫—tumor-bearers; NO2-production in macrophages stimulated with 10 ng/mL LPS during 24 h, nM/h/106 cells: □—in control mice and ■—in tumor-bearers. (b) Spontaneous NBT-test: ○—in macrophages from control mice, ⚫—from tumor-bearers; NBT-test in macrophages stimulated with 1 μg/mL TPA during 1 h: □—in control mice, ■—in tumor-bearers. Optical density was measured at nm. (c) Fluid-phase pinocytosis of neutral red during 1 h, optical density was measured at nm; □—macrophages from control mice, ■—from tumor-bearers. (d) 5′-nucleotidase activity was measured by colorimetric estimation of inorganic phosphate released from AMP at nm, nM/h/106 cells; □—macrophages from control mice, ■—from tumor-bearers. Each point represents the results of 4 independent experiments tested in quadriplicates (M ± SEM). Control mice received PBS. , , compared to control animals.
