Transplantable Subcutaneous Hepatoma 22a Affects Functional Activity of Resident Tissue Macrophages in Periphery
Figure 3
Hepatoma growth increases TSP-1 mRNA expression in peritoneal macrophages (a) and thymocytes (b). Total RNA was isolated from peritoneal cells or thymocytes from each mouse individually. Two μg of total RNA were used to perform semiquantitative (reverse-transcription) RT-PCR as described; mRNA levels specific for TSP-1 were normalized to β-actin. Each bar represents the average obtained from 8 mice (M ± SEM); , compared to control. (c) Representative RT-PCR analysis for TSP-1 mRNA expression in macrophages. (d) Representative RT-PCR analysis for TSP-1 mRNA expression in thymocytes. Thymocytes on the 35 day were not studied because of the thymic involution. NTB: nontumor bearing mice; TB: mice with hepatoma 22a.
