Research Article

Stable Differences in Intrinsic Mitochondrial Membrane Potential of Tumor Cell Subpopulations Reflect Phenotypic Heterogeneity

Figure 2

Subpopulations of cells within primary mammary carcinoma cell population exhibit stable differences in intrinsic ΔΨm. (a) The MCF7 cell line stained with jc-1 and analyzed by flow cytometry in FL-2. (b) The parental population of MCF7 and 83 subcloned cell lines were stained with JC-1, analyzed by flow cytometry, the Intrinsic ΔΨm each subcloned line was expressed relative to that of the unselected population of MCF7 cells and plotted as a frequency distribution. (c) 11 subcloned cell lines derived from SW480 cells were selected from further study. Mean and SEM of triplicate ΔΨm determinations made over approximately 3 months. Lower intrinsic ΔΨm are statistically different from higher intrinsic ΔΨm (Bonferroni's Multiple Comparison Test; ).
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978583.fig.002b
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