Stable Differences in Intrinsic Mitochondrial Membrane Potential of Tumor Cell Subpopulations Reflect Phenotypic Heterogeneity
Figure 5
Differences in the intrinsic ΔΨm of subcloned cell lines derived from a metastatic colonic tumor, but not from the paired primary colon tumor, are linked to phenotypic markers associated with invasion. (a) and (b) MMP7 secretion levels were quantified by ELISA in condition medium harvest from SW480 and SW620 Cells (a) and from 13 subcloned cell lines derived from SW480 cells (b) and normalized for cells/well by the MTT assay. Secretion levels in parental SW480 and derived subclones are plotted as a function of relative ΔΨm (b). (c) Fascin levels were determined in the SW480 and SW620 cell lines, and subclones derived from each of the cell lines, by immunoblotting normalized to actin. Reactions were quantified by densitometry and plotted as a function of relative ΔΨm.
