WIPI-1 Positive Autophagosome-Like Vesicles Entrap Pathogenic Staphylococcus aureus for Lysosomal Degradation

Figure 6

Pathogenic S. aureus SA113 induces GFP-WIPI-1 puncta formation and becomes entrapped in GFP-WIPI-1 positive autophagosome-like vesicles. According to Figures 4 and 5, GFP-WIPI-1 U2OS cells were infected with mCherry-expressing S. aureus SA113 in DMEM/FCS (a), DMEM (b), and EBSS (c) and analyzed (up to 2000 individual cells), representative images (2 h, DMEM/FCS) are shown ((d), scale bars: 20 μm), and the quantification of cells displaying GFP-WIPI-1 positive autophagosomal-like vesicles entrapping S. aureus SA113 is presented (e) ± SD (𝑛=2, each in duplicates).