Treatment with Y-27632, a ROCK Inhibitor, Increases the Proinvasive Nature of SW620 Cells on 3D Collagen Type 1 Matrix
Figure 5
SW620 cells were seeded at densities of 50 × 103 and 250 × 103 cells/cm2 onto 1.5 mg/mL collagen I gels. Scaffolds were treated with Y-27632 to study the impact of ROCK-1 inhibition on cell proliferation in a low- and high-density environment. Treatment with Y-27632 resulted in a modest increase in cell proliferation for cells seeded at 50 × 103 cells/cm2 and no significant change for those seeded at 250 × 103 cells/cm2. Furthermore, increasing cell density increased cell proliferation 2.5-fold for the untreated condition and 1.5-fold for those treated with Y-27632.
