Table 1: Kinetics of IDH isoforms as compared to homodimeric mutant enzymes with clinically relevant mutations. Unless specified “reductive”, forward reactions were measured. “n.d.”: not determined.

EnzymeOrganism/tissueMutationconditions isocitrate ( M) , 2-oxo-glutarate ( M) NADP+ ( M) (μmol·min−1·mg−1)Reference

IDH1Humanwt6519004944000 s−1[96]
HumanR132H370248438 s−1[96]
HumanR132HReductive2OG: 965n.dNADPH: 0.441000 s−1[96]
Humanwt6.4n.d.n.d.14[101]
HumanR132H1280n.d.n.d.0.8[101]
Rat liverwt120n.d.15070[102]

IDH2Porcinewt8.4n.d.5.643[103]
Porcinewt6n.d.540[51]
PorcineR133Q990n.d.1121[51]
Rat liverwt70n.d.6066[102]
Rat heartwt45804616[104]
Rat heartwtIschemic172504638[104]

IDH3HumanwtNo ADP2000n.d.NAD+: 6026[105]
Humanwt+1 mM ADP50n.d.n.d.n.d.[105]