Synergistic Modulation of Cellular Contractility by Mixed Extracellular Matrices
Figure 7
Morphometric analysis of MDA-MB-231 cells on mixed ECM surfaces. (a) Representative phase contrast images of MDA-MB-231 cells cultured for 24 hours on coverslips coated with 0.1 μg/cm2 collagen and fibronectin (Col, μg/cm2), 1.0 μg/cm2 collagen and fibronectin (Col, μg/cm2), and on fibroblast-derived matrix (FDM). Scale bar = 100 μm. (b) Box whisker plots of projected cell area on different mixed ECM surfaces. Cells spread to a lesser extent on FDMs. Maximal spreading was observed for Col, μg/cm2 (). (c) Box whisker plots of circularity measures on different mixed ECM surfaces. Cells were statistically more rounded on FDMs compared to the other conditions ().
