Early Decrease in Respiration and Uncoupling Event Independent of Cytochrome c Release in PC12 Cells Undergoing Apoptosis
Figure 2
Cytochrome release and nuclear apoptosis in STS-treated PC12 cells. (a) Triple labeling confocal immunofluorescence microscopy of cells untreated (Ctrl), 2 μM STS-treated for 3 h (STS 3 h), or 2 μM STS-treated for 6 h with 30 min zVADfmk (100 uM) pretreatment (zVAD-STS 6 h). Pattern of Hsp60 (red), cytochrome (green), merged patterns (yellow) and nuclear staining by SYTO-X (grey) of the same representative fields are shown. (b) Percentage of cytochrome releasing and apoptotic PC12 cells treated with STS for the indicated time. Data calculated in 3 independent experiments for each time point. About 1000 cells were considered for each experiment at each time point. Some SE are small and are within the symbols. Note that the ordinate scale starts at 50%. (c) Comparison between data shown in Figure 2(b) but expressed as decrease of cells with mitochondrially localized cytochrome and decrease of alive cells and data regarding decrease in respiration shown in Figure 1(b).
