Modulation of Tumor Cell Survival, Proliferation, and Differentiation by the Peptide Derived from Tenascin-C: Implication of β1-Integrin Activation
Table 1
Expression level of cell adhesion-relating molecules (VLA-4, 5, and syndecan-4) and induced cell adhesion or apoptosis by TNIIIA2 or Mg2+ administration in fresh AML cells from patients, peripheral blood cells from healthy adults, and hematopoietic tumor cell lines (the table was modified from [19]).
Cells
Expression (%)
Adhesion
Apoptosis
VLA-4
VLA-5
Syndecan-4
+TNIIIA2
+Mg2+
+TNIIIA2
+Mg2+
“Fresh AML cells”
Patient A
98.2
88.8
48.8
++
++
+
++
Patient B
97.5
98.5
9.5
−
+
−
++
“Peripheral blood cells”
Neutrophil
6.7
N.D.
3.2
−
−
−
−
Monocyte
48.6
N.D.
2.0
−
++
−
++
Lymphocyte
40.5
N.D.
4.4
−
++
−
+
“Cell lines”
B cell
Ramos
96.5
3.2
92.1
+++
++
+++
++
Raji
94.3
65.3
2.3
−
++
−
++
T cell
Jurkat
92.8
96.6
42.4
+++
++
+++
++
Erythroid
K562
9.3
97.2
66.7
+++
++
−
−
Myeloid
U937
98.1
98.2
87.5
+++
++
++
+++
HL60
99.8
99.7
75.3
+++
++
++
+
THP1
68.5
20.7
30.1
++
++
++
+
THP1 (+PMA)
10.5
18.7
99.2
++
++
−
−
Expression of VLA-4, 5 and syndecan-4 was evaluated by flowcytometric analysis.