PKC-Mediated ZYG1 Phosphorylation Induces Fusion of Myoblasts as well as of Dictyostelium Cells
Figure 2
Behavior of ZYG1 during cell fusion. GFP/ZYG1OE cells were starved for 2 h and fixed with methanol for 10 min. Pictures of their serial sections (1~3 : 4 μm thick) were taken under a confocal microscope. Pictures on the upper side show DIC images and those on the lower side the merged images of DIC, GFP, and DAPI staining. The color of DAPI staining is shown as red. In the DIC images (upper panels), the boundaries shown by white arrows are clear in all sections. On the other hand, the boundary shown by a yellow arrow is clearly observed in section 1, while the demarcation becomes unclear in sections 2 and 3. This indicates that the two cells are fusing around here (2, 3). In merged images (lower panels), GFP/ZYG1 fusion protein is localized at the region of cell fusion (2, 3; yellow arrows). Nuclei become yellow by merging GFP with DAPI staining. Bar: 20 μm.
