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International Journal of Cell Biology
Volume 2012, Article ID 657423, 11 pages
http://dx.doi.org/10.1155/2012/657423
Research Article

PKC-Mediated ZYG1 Phosphorylation Induces Fusion of Myoblasts as well as of Dictyostelium Cells

1Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan
2Zoologisch Institute, Ludwig-Maximilians Universitat, 80333 München, Germany
3Central Research Laboratory, Shiga University of Medical Science, Otsu, Shiga 520-2192, Japan
4Department of Physiology, Shiga University of Medical Science, Otsu, Shiga 520-2192, Japan

Received 12 June 2011; Revised 1 October 2011; Accepted 1 October 2011

Academic Editor: Andre Van Wijnen

Copyright © 2012 Aiko Amagai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1: Behavior of GFP/ZYG1 protein in intact GFP/ZYG1OE cells.

Figure S2: Detection of GFP protein and GFP/ZYG1 fusion protein by the anti-GFP antibody.

Figure S3: Acquisition of the fusion competence in myoblasts transfecting ha/mzyg1 fusion gene.

Figure S4: Mitotic division observed in C2C12 cells.

Figure S5: Comparison of intact C2C12 cells with those transfected with the pIRES-AcGFP vector.

Figure S6: GFP images merged with DIC images in C2C12 cells transfected with the pIRES2-AcGFP vector (A) and in C2C12 cells transfected with the pIRES2-AcGFP vector containing the mzyg1 gene (B).

  1. Supplementary Figures