Partitioning and Exocytosis of Secretory Granules during Division of PC12 Cells

Figure 1

Ultrastructural analysis of the distribution of SGs in interphase and metaphase PC12 cells. (a) A typical interphase PC12 cell. The majority of SGs (size range of 80 to 150 nm) is peripherally localized (orange arrows). (b) A PC12 cell in metaphase. Almost all SGs are homogeneously distributed in the cytoplasm (yellow arrows), whereas peripheral SGs are rarely observed (orange arrows). Magnifications of the indicated regions in the main images (boxes in (A) and (B)) are shown on the right ((A₁), (A₂), (B₁), and (B₂)). The red dashed lines indicate the cell boundaries. N, nucleus; CH, chromosomes. Scale bars, main images 5 μm, magnified images, 500 nm.