Zinc Protoporphyrin Upregulates Heme Oxygenase-1 in PC-3 Cells via the Stress Response Pathway
Induction of HO-1 by ZnPP in PC-3 for various lengths of time. PC-3 cells were treated with 10 μM ZnPP for 0.5, 1, 4, 16, 24, and 48 h (lanes 2, 3, 4, 5, 6, and 7, resp.). Control cells were treated with equal amount of DMSO for 48 h (lane 1). (a) Western blot analysis was done as described in Section 2, using specific antibodies against β-actin and HO-1. Immunoreactive protein bands detected by WesternDot 625 appeared as fluorescent bands. (b) RT-PCR analysis using gene-specific primers. PCR fragments were visualized by ethidium bromide staining.
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