Fibrin and Collagen Differentially but Synergistically Regulate Sprout Angiogenesis of Human Dermal Microvascular Endothelial Cells in 3-Dimensional Matrix

Figure 7

Effect of GPenGRGDSPCA peptides (RGD (VN)) on sprout angiogenesis of HDMEC in fibrin. HDMEC cultured on microcarrier beads were incubated with various doses of RGD (VN) (12.5 micro M–100 micro M) peptides at RT for 1 hr. The EC-beads were added to fibrinogen solution with presence of VEGF (30 ng/mL) and bFGF (25 ng/mL) and then polymerized with thrombin. RGD (VN) significantly inhibited sprout angiogenesis of HDMEC in fibrin compared to GRGESP(RGE) control peptides and nonspecific integrin antagonist cyclic RGD peptide (GRGDNP).