Research Article

Mitochondrial Complex I Inhibitors and Forced Oxidative Phosphorylation Synergize in Inducing Cancer Cell Death

Figure 6

Piericidin A and low glucose synergize in inducing death also of pancreatic and lung cancer cells. Viable cell count using Trypan Blue was performed after treatment with 10 nM piericidin A in different growth conditions. (a)-(b) MIA PaCa-2 (pancreatic, A) and A549 (lung, B) cancer cells were cultured in 25 mM glucose and counted after 8-hour treatment. (c)-(d) MIA PaCa-2 (c) and A549 (d) cells were cultured in 1 mM glucose and treated with piericidin A, 10  M FSK, or FSK plus piericidin A. For the cotreatment experiments, cells were pretreated with FSK for 1 hour and then piericidin A was added for 8 hours (see Figure 2(c), as example). After treatment cell count was performed. All data represent the average of at least three independent experiments (±s.d.); , (Student’s   -test).
243876.fig.006a
(a)
243876.fig.006b
(b)
243876.fig.006c
(c)
243876.fig.006d
(d)