Modulation of Vascular Cell Function by Bim Expression
Bim−/− retinal endothelial cells and pericytes demonstrate increased migration. Cell migration was determined by the scratch wounding of retinal endothelial cells (panel (a)) or pericytes (panel (b)) monolayers, and wound closure was monitored using a phase microscope in digital format at low magnification (×40). A representative experiment is shown here. Please note that wild-type vascular cells migrate slower than Bim−/− cells. The quantitative assessment of the data is shown in panels (c) and (d). Transwell assays were performed with wild-type and Bim−/− retinal endothelial cells (panel (e)) and pericytes (panel (f)). These experiments were repeated twice with similar results (***).