Quantifying Changes in the Cellular Thiol-Disulfide Status during Differentiation of B Cells into Antibody-Secreting Plasma Cells
Figure 2
Cell density does not affect protein thiol-disulfide status in resting B cells. Experiments were performed on two independent cultures. (a) Growth curve of 1.29μ + lymphoma cells cultured in suspension as described in [13]. Each day a fraction of the culture was harvested for global quantification of the thiol-disulfide status, as illustrated in Figure 1. The graph shows cell densities at the times of sample preparation. (b) Distribution of protein sulfhydryl equivalents in samples harvested on days 1–4. SH/aa values for PSH (●) and PSox (○) are shown.
