Matrix Gla Protein Binds to Fibronectin and Enhances Cell Attachment and Spreading on Fibronectin
MGP and fibronectin localization in the embryonic rat kidney. (a)–(c) serial section of E20 rat kidney in which fibronectin is localized with mouse-anti-fibronectin and Vectastain ABC elite universal peroxidase-diaminobenzidine and counterstained with methyl green. (d)–(f) Serial section of E20 rat kidney in which MGP localized with rabbit-anti-MGP, Vectastain ABC elite universal peroxidase diaminobenzidine and counterstained with methyl green. A and D are low power images of the same region of kidney taken with the 6.3x objective. U is the lumen of the developing ureter, and G is a developing glomerulus. Fibronectin localized in B and C taken with a 40x oil immersion objective. U is the lumen of the developing ureter. Arrows indicate tubules identical to those shown in the serial section. MGP localized in E and F, image of equivalent fields as B and C, taken at the same magnification with a 40x objective. U is the developing ureter. Arrows indicate intensely stained tubules for comparison.