International Journal of Cell Biology / 2014 / Article / Fig 6

Research Article

Matrix Gla Protein Binds to Fibronectin and Enhances Cell Attachment and Spreading on Fibronectin

Figure 6

MGP and fibronectin localization in the embryonic rat kidney. (a)–(c) serial section of E20 rat kidney in which fibronectin is localized with mouse-anti-fibronectin and Vectastain ABC elite universal peroxidase-diaminobenzidine and counterstained with methyl green. (d)–(f) Serial section of E20 rat kidney in which MGP localized with rabbit-anti-MGP, Vectastain ABC elite universal peroxidase diaminobenzidine and counterstained with methyl green. A and D are low power images of the same region of kidney taken with the 6.3x objective. U is the lumen of the developing ureter, and G is a developing glomerulus. Fibronectin localized in B and C taken with a 40x oil immersion objective. U is the lumen of the developing ureter. Arrows indicate tubules identical to those shown in the serial section. MGP localized in E and F, image of equivalent fields as B and C, taken at the same magnification with a 40x objective. U is the developing ureter. Arrows indicate intensely stained tubules for comparison.
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