Carboxymethyl Hyaluronan-Stabilized Nanoparticles for Anticancer Drug Delivery

Figure 2

Physical characterization of nCa using X-ray diffraction and transmission electron microscopy. (a) XRD spectra of nCa suspension (nCa wet), lyophilized nCa (nCa dry), and lyophilized CaP without stabilizer added during precipitation (microCaP dry). Hydroxyapatite standard (JCPDS, #09-0432, bars) is shown for comparison. nCa both wet and dry has a major broad peak corresponding to the major peaks of hydroxyapatite. MicroCaP pattern has major peaks characteristic of brushite (peaks denoted by open circles). MicroCaP was precipitated without a stabilizer and has larger crystalline particles (narrow peaks). With the CMHA stabilizer present the crystallization is halted, depicted by broad peaks with little long range order. (b) TEM image of nCaCDDP showing particles 20–50 nm in diameter clustered into larger, 200 nm, particles due to drying prior to imaging.

(a)

(b)