Carboxymethyl Hyaluronan-Stabilized Nanoparticles for Anticancer Drug Delivery

Figure 5

Flow cytometry data for the BT-474 and BT-47 cells demonstrates appropriate CD44 expression for the studies. The numbers in the corner of each quadrant are the percentage of positive cells within the quadrant. (a) BT-474 unstained control, (b) BT-474 isotype control, (c) BT-474 stained cells with CD44, Alexa Fluor 647, and CD24, PE-Cy7. BT-474 cells are CD4/CD2. These cells served as the negative control for CD44 targeting, because they lack CD44 expression. (d) BT-47 unstained control, (e) BT-47 isotype control, (f) BT-47 stained cells with CD44, Alexa Fluor 647, and CD24, PE-Cy7. BT-47 cells are CD44⁺/CD2. These cells were tested to investigate CD44 targeting, as they have high CD44 expression.

(a)  

(b)

(c)

(d)

(e)

(f)